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biotinylated primary antibody against human erbb4  (R&D Systems)


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    Structured Review

    R&D Systems biotinylated primary antibody against human erbb4
    Key resources table
    Biotinylated Primary Antibody Against Human Erbb4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+primary+antibody+against+human+erbb4/Human+ErbB4%2FHer4+Biotinylated+Antibody/pmc10993865-829-7-14
    Average 92 stars, based on 1 article reviews
    biotinylated primary antibody against human erbb4 - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "Human pallial MGE-type GABAergic interneuron cell therapy for chronic focal epilepsy"

    Article Title: Human pallial MGE-type GABAergic interneuron cell therapy for chronic focal epilepsy

    Journal: Cell stem cell

    doi: 10.1016/j.stem.2023.08.013

    Key resources table
    Figure Legend Snippet: Key resources table

    Techniques Used: Control, Plasmid Preparation, Recombinant, Software

    (A,B) Simplified schematics of neuronal subtypes derived from the MGE progenitor domain (FOXG1+ DLX1/2+ NKX2-1+), including GABAergic pallial interneurons (pINs) that migrate to the cortex and hippocampus (HC), as well as multiple lineages that remain in the subpallium. The latter include striatal GABAergic and cholinergic INs, cholinergic projection neurons (PN) of the basal telencephalon, and GABAergic PNs of the globus pallidus. Listed genes expressed in the immature neurons play critical roles in the specification of the indicated neuronal subtypes. (C,D) Representative ICC images of unsorted and sorted lot 1 after cryopreservation and thaw show expression of MGE pIN markers (LHX6, MAFB, MAF, ERBB4), and cholinergic neurons (ISL1). (E) Protein quantification across independent lots (n=10 to 18). (F,G) Quantification of GABA (F) and Acetylcholine (G) neurotransmitters in the culture supernatant from independent pIN lots (n=8), undifferentiated hESCs (n=1) and spinal motor neurons (n=1). (H) Cell viability post-thaw from independent pIN lots (n=16 unsorted/sorted pairs). In E-H, each dot is an average of technical replicates (2-3) from independently manufactured lots. All data are expressed as a mean ± SEM.
    Figure Legend Snippet: (A,B) Simplified schematics of neuronal subtypes derived from the MGE progenitor domain (FOXG1+ DLX1/2+ NKX2-1+), including GABAergic pallial interneurons (pINs) that migrate to the cortex and hippocampus (HC), as well as multiple lineages that remain in the subpallium. The latter include striatal GABAergic and cholinergic INs, cholinergic projection neurons (PN) of the basal telencephalon, and GABAergic PNs of the globus pallidus. Listed genes expressed in the immature neurons play critical roles in the specification of the indicated neuronal subtypes. (C,D) Representative ICC images of unsorted and sorted lot 1 after cryopreservation and thaw show expression of MGE pIN markers (LHX6, MAFB, MAF, ERBB4), and cholinergic neurons (ISL1). (E) Protein quantification across independent lots (n=10 to 18). (F,G) Quantification of GABA (F) and Acetylcholine (G) neurotransmitters in the culture supernatant from independent pIN lots (n=8), undifferentiated hESCs (n=1) and spinal motor neurons (n=1). (H) Cell viability post-thaw from independent pIN lots (n=16 unsorted/sorted pairs). In E-H, each dot is an average of technical replicates (2-3) from independently manufactured lots. All data are expressed as a mean ± SEM.

    Techniques Used: Derivative Assay, Expressing

    Key resources table
    Figure Legend Snippet: Key resources table

    Techniques Used: Control, Plasmid Preparation, Recombinant, Software

    Related Articles

    Suspension:

    Article Title: Human pallial MGE-type GABAergic interneuron cell therapy for chronic focal epilepsy
    Article Snippet: .. Single cell suspension was incubated with a biotinylated primary antibody against human ERBB4 (BAF1131 R&D Systems), followed by incubation with Anti-Biotin MicroBeads (130-105-637, Miltenyi Biotec), and magnetic sorting was performed on the CliniMACS according to manufacturer instructions (Miltenyi Biotec). .. Unsorted (pre-sort) and sorted (post-positive selection) cell populations were cryopreserved using the CryoMed controlled-rate freezer (Fisher Scientific), before further storage in vapor-phase liquid nitrogen.

    Article Title: Human pallial MGE-type GABAergic interneuron cell therapy for chronic focal epilepsy.
    Article Snippet: Cell batch processing and cryopreservation At the end of the differentiation process, cells were harvested and dissociated to single cells using TrypLE Select Enzyme and Benzonase (1/10000; Sigma Aldrich). .. Single cell suspension was incubated with a biotinylated primary antibody against human ERBB4 (BAF1131 R&D Systems), followed by incubation with Anti-Biotin MicroBeads (130-105- 637, Miltenyi Biotec), and magnetic sorting was performed on the CliniMACS according to manufacturer instructions (Miltenyi Biotec). .. Libraries were prepared using CleanPlex TP53 Panel (Paragon Genomics) and sequenced on the Illumina platform and analyzed by ACGT Inc for TP53 mutations recurrently acquired in human pluripotent stem cells during in vitro culture.103 The characterizations demonstrated: a. no genetic abnormality occurs during ESC expansion or following the interneuron production process from ESCs at the maximum limit of in vitro cultivation; and b. consistent production of the intended pallial interneuron cell product at the maximum limit of in vitro cultivation.

    Incubation:

    Article Title: Human pallial MGE-type GABAergic interneuron cell therapy for chronic focal epilepsy
    Article Snippet: .. Single cell suspension was incubated with a biotinylated primary antibody against human ERBB4 (BAF1131 R&D Systems), followed by incubation with Anti-Biotin MicroBeads (130-105-637, Miltenyi Biotec), and magnetic sorting was performed on the CliniMACS according to manufacturer instructions (Miltenyi Biotec). .. Unsorted (pre-sort) and sorted (post-positive selection) cell populations were cryopreserved using the CryoMed controlled-rate freezer (Fisher Scientific), before further storage in vapor-phase liquid nitrogen.

    Article Title: Human pallial MGE-type GABAergic interneuron cell therapy for chronic focal epilepsy.
    Article Snippet: Cell batch processing and cryopreservation At the end of the differentiation process, cells were harvested and dissociated to single cells using TrypLE Select Enzyme and Benzonase (1/10000; Sigma Aldrich). .. Single cell suspension was incubated with a biotinylated primary antibody against human ERBB4 (BAF1131 R&D Systems), followed by incubation with Anti-Biotin MicroBeads (130-105- 637, Miltenyi Biotec), and magnetic sorting was performed on the CliniMACS according to manufacturer instructions (Miltenyi Biotec). .. Libraries were prepared using CleanPlex TP53 Panel (Paragon Genomics) and sequenced on the Illumina platform and analyzed by ACGT Inc for TP53 mutations recurrently acquired in human pluripotent stem cells during in vitro culture.103 The characterizations demonstrated: a. no genetic abnormality occurs during ESC expansion or following the interneuron production process from ESCs at the maximum limit of in vitro cultivation; and b. consistent production of the intended pallial interneuron cell product at the maximum limit of in vitro cultivation.

    other:

    Article Title: Human pallial MGE-type GABAergic interneuron cell therapy for chronic focal epilepsy.
    Article Snippet: Libraries were prepared using CleanPlex TP53 Panel (Paragon Genomics) and sequenced on the Illumina platform and analyzed by ACGT Inc for TP53 mutations recurrently acquired in human pluripotent stem cells during in vitro culture.103 The characterizations demonstrated: a. no genetic abnormality occurs during ESC expansion or following the interneuron production process from ESCs at the maximum limit of in vitro cultivation; and b. consistent production of the intended pallial interneuron cell product at the maximum limit of in vitro cultivation.

    Single Cell:

    Article Title: Human pallial MGE-type GABAergic interneuron cell therapy for chronic focal epilepsy
    Article Snippet: .. Single cell suspension was incubated with a biotinylated primary antibody against human ERBB4 (BAF1131 R&D Systems), followed by incubation with Anti-Biotin MicroBeads (130-105-637, Miltenyi Biotec), and magnetic sorting was performed on the CliniMACS according to manufacturer instructions (Miltenyi Biotec). .. Unsorted (pre-sort) and sorted (post-positive selection) cell populations were cryopreserved using the CryoMed controlled-rate freezer (Fisher Scientific), before further storage in vapor-phase liquid nitrogen. . Immunocytochemistry Thawed cells were counted using the NC-200 cell counter (Chemometec) and seeded into 384-well plates coated with Poly-L-Ornithine/Fibronectin/Laminin (PO/Fib/Lam) at ~4.0E5-4.3E5 cells/cm 2 .

    Article Title: Human pallial MGE-type GABAergic interneuron cell therapy for chronic focal epilepsy.
    Article Snippet: Cell batch processing and cryopreservation At the end of the differentiation process, cells were harvested and dissociated to single cells using TrypLE Select Enzyme and Benzonase (1/10000; Sigma Aldrich). .. Single cell suspension was incubated with a biotinylated primary antibody against human ERBB4 (BAF1131 R&D Systems), followed by incubation with Anti-Biotin MicroBeads (130-105- 637, Miltenyi Biotec), and magnetic sorting was performed on the CliniMACS according to manufacturer instructions (Miltenyi Biotec). .. Libraries were prepared using CleanPlex TP53 Panel (Paragon Genomics) and sequenced on the Illumina platform and analyzed by ACGT Inc for TP53 mutations recurrently acquired in human pluripotent stem cells during in vitro culture.103 The characterizations demonstrated: a. no genetic abnormality occurs during ESC expansion or following the interneuron production process from ESCs at the maximum limit of in vitro cultivation; and b. consistent production of the intended pallial interneuron cell product at the maximum limit of in vitro cultivation.



    Similar Products

    biotinylated primary antibody against human erbb4  (R&D Systems)
    92
    R&D Systems biotinylated primary antibody against human erbb4
    Key resources table
    Biotinylated Primary Antibody Against Human Erbb4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+primary+antibody+against+human+erbb4/Human+ErbB4%2FHer4+Biotinylated+Antibody/pmc10993865-829-7-14
    Average 92 stars, based on 1 article reviews
    biotinylated primary antibody against human erbb4 - by Bioz Stars, 2026-09
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Key resources table

    Journal: Cell stem cell

    Article Title: Human pallial MGE-type GABAergic interneuron cell therapy for chronic focal epilepsy

    doi: 10.1016/j.stem.2023.08.013

    Figure Lengend Snippet: Key resources table

    Article Snippet: Single cell suspension was incubated with a biotinylated primary antibody against human ERBB4 (BAF1131 R&D Systems), followed by incubation with Anti-Biotin MicroBeads (130-105-637, Miltenyi Biotec), and magnetic sorting was performed on the CliniMACS according to manufacturer instructions (Miltenyi Biotec).

    Techniques: Control, Plasmid Preparation, Recombinant, Software

    (A,B) Simplified schematics of neuronal subtypes derived from the MGE progenitor domain (FOXG1+ DLX1/2+ NKX2-1+), including GABAergic pallial interneurons (pINs) that migrate to the cortex and hippocampus (HC), as well as multiple lineages that remain in the subpallium. The latter include striatal GABAergic and cholinergic INs, cholinergic projection neurons (PN) of the basal telencephalon, and GABAergic PNs of the globus pallidus. Listed genes expressed in the immature neurons play critical roles in the specification of the indicated neuronal subtypes. (C,D) Representative ICC images of unsorted and sorted lot 1 after cryopreservation and thaw show expression of MGE pIN markers (LHX6, MAFB, MAF, ERBB4), and cholinergic neurons (ISL1). (E) Protein quantification across independent lots (n=10 to 18). (F,G) Quantification of GABA (F) and Acetylcholine (G) neurotransmitters in the culture supernatant from independent pIN lots (n=8), undifferentiated hESCs (n=1) and spinal motor neurons (n=1). (H) Cell viability post-thaw from independent pIN lots (n=16 unsorted/sorted pairs). In E-H, each dot is an average of technical replicates (2-3) from independently manufactured lots. All data are expressed as a mean ± SEM.

    Journal: Cell stem cell

    Article Title: Human pallial MGE-type GABAergic interneuron cell therapy for chronic focal epilepsy

    doi: 10.1016/j.stem.2023.08.013

    Figure Lengend Snippet: (A,B) Simplified schematics of neuronal subtypes derived from the MGE progenitor domain (FOXG1+ DLX1/2+ NKX2-1+), including GABAergic pallial interneurons (pINs) that migrate to the cortex and hippocampus (HC), as well as multiple lineages that remain in the subpallium. The latter include striatal GABAergic and cholinergic INs, cholinergic projection neurons (PN) of the basal telencephalon, and GABAergic PNs of the globus pallidus. Listed genes expressed in the immature neurons play critical roles in the specification of the indicated neuronal subtypes. (C,D) Representative ICC images of unsorted and sorted lot 1 after cryopreservation and thaw show expression of MGE pIN markers (LHX6, MAFB, MAF, ERBB4), and cholinergic neurons (ISL1). (E) Protein quantification across independent lots (n=10 to 18). (F,G) Quantification of GABA (F) and Acetylcholine (G) neurotransmitters in the culture supernatant from independent pIN lots (n=8), undifferentiated hESCs (n=1) and spinal motor neurons (n=1). (H) Cell viability post-thaw from independent pIN lots (n=16 unsorted/sorted pairs). In E-H, each dot is an average of technical replicates (2-3) from independently manufactured lots. All data are expressed as a mean ± SEM.

    Article Snippet: Single cell suspension was incubated with a biotinylated primary antibody against human ERBB4 (BAF1131 R&D Systems), followed by incubation with Anti-Biotin MicroBeads (130-105-637, Miltenyi Biotec), and magnetic sorting was performed on the CliniMACS according to manufacturer instructions (Miltenyi Biotec).

    Techniques: Derivative Assay, Expressing

    Key resources table

    Journal: Cell stem cell

    Article Title: Human pallial MGE-type GABAergic interneuron cell therapy for chronic focal epilepsy

    doi: 10.1016/j.stem.2023.08.013

    Figure Lengend Snippet: Key resources table

    Article Snippet: Single cell suspension was incubated with a biotinylated primary antibody against human ERBB4 (BAF1131 R&D Systems), followed by incubation with Anti-Biotin MicroBeads (130-105-637, Miltenyi Biotec), and magnetic sorting was performed on the CliniMACS according to manufacturer instructions (Miltenyi Biotec).

    Techniques: Control, Plasmid Preparation, Recombinant, Software